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Staining: Metachromasia
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Slide 3: This plastic embedded section (of cartilage on the right and adjacent connective tissue) is thicker than Slide 2 and so stains more deeply. Metachromasia is the property of some dyes like toluidine blue which change color when bound to macromolecules with many repeating subunits such as the carbohydrates of cartilage matrix (M). As you can see, the metachromatic color of toluidine blue is purple.