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Male Reproductive System

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Slide 1: Testis, rabbit, sections of two seminiferous tubules. Note the stages of spermatogenesis, Sertoli cells and myoid cells.  

Slide 2: Immature monkey tubules with gonia, Sertoli cells (ones with nucleoli), Leydig cells, myoid cells. Which of the cells identified in the preceeding slide are missing here?  

Slide 3: Adult human. Note the Leydig cells (eosinophilic interstitial cells), myoid cells, portions of 3 seminiferous tubules, gonia, spermatocytes, and spermatids (oval shaped dark staining nuclei).  

Slide 4: Testis, plastic embedded section. Leydig cells. Note the lipid droplets in these cells indicating stored cholesterol for steroid synthesis (testosterone), myoid cells, and the small amount of connective tissue between the tubules.  

Slide 5: Part of two seminiferous tubules, monkey,plastic embedded section. Find the Sertoli cells, spermatids (uniformly dense nuclei). Nuclei of spermatids are just beginning to become condensed, they are becoming oval-shaped.  

Slide 6: Basal portion of two tubules, plastic embedded section. Note gonia, primary spermatocytes (which type of spermatogonia do these arise from?- ans B spermatogonia), early and late spermatids, Sertoli cells, and myoid cells. Can you find 3 spermatogonia, maybe 5 Sertoli cells here?  

Slide 7: Seminiferous tubule, plastic embedded section. Note the stages of spermatogenesis and spermiogenesis.  

Slide 8: Spermiogenesis in progress, plastic embedded section. Locate the developing midpiece and annulus of a developing late spermatid. Which spermatids are more mature here, the lighter or darker staining nuclei? Why? (Ans. darker, because of the condensation of the chromatin).  

Slide 9: Seminiferous tubule. Note the almost mature spermatozoa towards the lumen.  

Slide 10: Find the 3 segments of straight tubule.  

Slide 11: Testis, monkey. The mediastinum has straight tubules (note the simple columnar epithelium). The seminiferous tubules are not well preserved and have shrunken away from the basal lamina.  

Slide 12: Rete testis and efferent ductules, very low power. See the next image to examine the epithelium of each.  

Slide 13: Thick paraffin section with rete testis at the top of the field and an efferent ductule at the bottom of the field. Compare their epithelia.  

Slide 14: Efferent ductules (irregular lumen) adjacent to epididymis (smoother lumen), low power, human. See the next 2 slides for higher power images.  

Slide 15: Efferent ductules and epididymis. Part of slide #15. The efferent ductules have an irregular luminal contour because of adjacent patches of cuboidal and columnar cells and some cells with cilia (the only motile cilia in the entire male reproductive system).  

Slide 16: Efferent ductule and epididymis. Compare their epithelia. This section of epididymis is near the head (caput) because the layer of smooth muscle around it is rather thin.  

Slide 17: Adjacent sections of efferent ductule and epididymis. The epithelium all the way to the prostatic urethra is pseudostratified columnar with sterocilia. Can you tell the difference between cilia and stereocilia here. (Ans: no, only with EM).  

Slide 18: Epididymis, plastic embedded section, Monkey. Note the small basal cells that make this a pseudostratified epithelium.  

Slide 19: Ductus deferens, low power. This is part of the spermatic cord, along with the testicular artery, pampiniform plexus of veins and nerves; plastic embedded section. Note the very thick smooth musculature.  

Slide 20: Vas deferens, plastic embedded section. Can you define the muscle layers, (2 longitudinal ones and a central, circular smooth muscle layer).  

Slide 21: Vas deferens, toluidine blue-acid fuchsin stain; plastic embedded section. The basal cells of the pseudostratified columnar epithelium are quite numerous. Note: We don't have a slide of the ampulla of the vas, but it would have a dilated lumen and circular or oblique smooth muscle only, not the three layers of the rest of the vas).  

Slide 22: Ureter and seminal vesicles, blood vessels, lymphatics and a small part of the vas (bottom of the field), low power.  

Slide 23: Seminal vesicles from normal and castrated (no testosterone) rats. Note the smooth muscle surrounding the whole vesicle, but not extending into the lamina propria of the mucosal folds. Compare the castrate (right side) with the normal.  

Slide 24: Monkey, higher power. Note the recesses, luminal secretion and that the smooth muscle does not extend into the lamina propria.  

Slide 25: Man. Compare with gall bladder.  

Slide 26: Plastic embedded section, low power. The connective tissue is light pink, darker pink is smooth muscle, and intensely bright pink is blood within vessels. See the next image for a higher power view of the epithelium.  

Slide 27: Plastic embedded section, monkey. Seminal vesicle cut parallel to the muscle. Note the pseudostratified columnar epithelium.  

Slide 28: Prostate from an older male, low power. Some of the lumina contain concretions. Note the smooth muscle in the lamina propria.  

Slide 29: Very low power. Prostatic urethra, periurethral glands, prostate proper, some seminal vesicles or ampulla, human. Find the tear in the section (it looks like a lumen but isn't).  

Slide 30: Prostate and periurethral glands (note smooth muscle), thick section, young individual.  

Slide 31: Young. Note the fibromuscular stroma. The epithelium is pseudostratified columnar, but it is difficult to tell by this field.  

Slide 32: Prostate gland, human. From an older individual note the concretions and the pseudostratified columnar epithelium.  

Slide 33: Young monkey, plastic embedded section. Here the epithelium is easier to characterize and supranuclear secretory granules are seen Compare with slides #25 and #26.  

Slide 34: Old human prostate, plastic embedded section. Compare the epithelium with slide #34 and note the lamellated concretion.  

Slide 35: Prostatic urethra, low power. Note the epithelium, it can be difficult to diagnose (impossible in this image).  

Slide 36: x.s., low power, near the glans. Note the obliquely sectioned skin of the prepuce. How can you tell which level this section of the penis is? (hint: cavernous bodies).  

Slide 37: Corpus spongiosum, urethra, glands of Littre. Lymphoid nodule.  

Slide 38: Penile urethra and glands of Littre. Note the stratified columnar epithelium (higher mag. on slide 40).  

Slide 39: Epithelium of the urethra (peudostratified? or stratified columnar- hard to tell) and mucous intraepithelial gland.  

Slide 40: Cross section of the corpus spongiosum and urethra. Note the filled blood spaces.  

Slide 41: Erectile blood spaces lined by endothelium of the spongiosum with smooth muscle bundles. We do not have slides of the following, but consider these additional points: Glans and prepuce with sebaceous glands not associated with hair follicles, Cowper's (bulbourethral) mucous glands.

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