James' Unwanted Blood Clots
The antithrombin III test measures how well James' plasma inhibits thrombin.
This part of the tutorial illustrates aspects of enzyme kinetics.
In the antithrombin III assay, thrombin catalyzes the reaction:
H-D-HHT-L-Ala-L-Arg-pNA (colorless) → H-D-HHT-L-Ala-L-Arg + pNA (colored)
For this substrate, thrombin has Km=3.24 μM
Question:
The company that manufactures the assay kit directs people to use the thrombin substrate at a concentration of 180 μM.
what would happen if you used half the concentration of substrate (90 μM)?
- there would be no reaction
- the reaction would proceed very slowly (less than 10% of the normal rate)
- the reaction would proceed at about half of the normal rate
- very little change: the reaction would proceed at >90% of the normal rate
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